Working Safely with Vaccinia Virus: Laboratory Technique and the Role of Vaccinia Vaccination
Stuart N. Isaacs

Construction and Isolation of Recombinant Vaccinia Virus Using Genetic Markers
María M. Lorenzo, Inmaculada Galindo, and Rafael Blasco

Construction of Recombinant Vaccinia Virus: Cloning into the Thymidine Kinase Locus
Chelsea M. Byrd and Dennis E. Hruby

Transient and Inducible Expression of Vaccinia/T7 Recombinant Viruses
Mohamed Ragaa Mohamed and Edward G. Niles

Construction of Recombinant Vaccinia Viruses Using Leporipoxvirus-Catalyzed Recombination and Reactivation of Orthopoxvirus DNA, Xiao-Dan Yao and David H. Evans

Construction of cDNA Libraries in Vaccinia Virus
Ernest S. Smith, Shuying Shi, and Maurice Zauderer

Construction and Isolation of Recombinant MVA
Caroline Staib, Ingo Drexler, and Gerd Sutter

Growing Poxviruses and Determining Virus Titer
Girish J. Kotwal and Melissa-Rose Abrahams

Rapid Preparation of Vaccinia Virus DNA Template for Analysis and Cloning by PCR
Rachel L. Roper

Orthopoxvirus Diagnostics
Hermann Meyer, Inger K. Damon, and Joseph J. Esposito

An In Vitro Transcription System for Studying Vaccinia Virus Early Genes
Steven S. Broyles and Marcia Kremer

An In Vitro Transcription System for Studying Vaccinia Virus Late Genes
Cynthia F. Wright

Studying Vaccinia Virus RNA Processing In Vitro
Paul D. Gershon

Methods for Analysis of Poxvirus DNA Replication
Paula Traktman and Kathleen Boyle

Studying the Binding and Entry of the Intracellular and Extracellular Enveloped Forms of Vaccinia Virus
Mansun Law and Geoffrey L. Smith

Pox, Dyes, and Videotape: Making Movies of GFP-Labeled Vaccinia Virus
Brian M. Ward

Interaction Analysis of Viral Cytokine-Binding Proteins Using Surface PlasmonResonance
Bruce T. Seet and Grant McFadden

Monitoring of Human Immunological Responses to Vaccinia Virus
Richard Harrop, Matthew G. Ryan, Hana Golding, Irina Redchenko, and Miles W. Carroll

Vaccinia Virus as a Tool for Immunologic Studies
Nia Tatsis, Gomathinayagam Sinnathamby, and Laurence C. Eisenlohr

Mouse Models for Studying Orthopoxvirus Respiratory Infections
Jill Schriewer, R. Mark L. Buller, and Gelita Owens

Viral Glycoprotein-Mediated Cell Fusion Assays Using Vaccinia Virus Vectors
Katharine N. Bossart and Christopher C. Broder

Use of Dual-Recombinant Vaccinia Virus Vectors to Assay Viral Glycoprotein-Mediated Fusion with Transfection-Resistant Primary Cell Targets
Yanjie Yi, Anjali Singh, Joanne Cutilli, and Ronald G. Collman

Poxvirus Bioinformatics
Chris Upton

Preparation and Use of Molluscum Contagiosum Virus from Human Tissue Biopsy Specimens
Nadja V. Melquiot and Joachim J. Bugert

Index