Optical phase microscopy is a technique used in fields such as disease diagnosis and monitoring or at the nanoscale for structural analysis. Its advantage is allowing the viewing of unstained biological specimens using the light path delay differences within microscopic objects. Written by global experts, this book provides a comprehensive reference to phase microscopy and nanoscopy techniques for biomedical applications, including differential interference contrast (DIC) microscopy, phase contrast microscopy, digital holographic microscopy, optical coherence tomography, tomographic phase microscopy, spectral-domain phase detection, and nanoparticle usage for phase nanoscopy. The Editors show biomedical and optical engineers how to create and use phase microscopy for visualizing unstained specimens and support the coverage with applied content and examples on designing systems and interpreting results in standard bioscience and nanoscience applications. It will be of strong interest to engineers designing optical systems for use in cell biology, medical diagnosis, and disease monitoring, as well as the study and imaging of nanometric structures within biological specimens.

Preface Natan T. Shaked, Zeev Zalevskey and Lisa L. Satterwhite

1. Phase contrast microscopy and differential interference contrast microscopy

Chapter 1

Principles of phase contrast microscopy

Sam A. Johnson

Editor: Lisa L. Satterwhite

Chapter 2

Differential interference contrast (DIC) microscopy

Michael Shribak Editor: Lisa L. Satterwhite

Chapter 3

Long term phase contrast imaging of untransformed human cells

Yumi Uetake and Greenfield Sluder

Editor: Lisa L. Satterwhite

Chapter 4

Phase imaging of plant cells and tissues

Vassilios Sarfis Editor: Zeev Zalevsky

2. Digital holographic phase microscopy

Chapter 5

Measuring biophysical parameters of living cells with digital holographic microscopy

Benjamin Rappaz, Christian Depeursinge and Pierre Marquet

Editor: Natan T. Shaked

Chapter 6

New digital holographic microscopy setups and techniques for obtaining multi-focus quantitative phase imaging of living cells

Björn Kemper, Patrik Langehanenberg and Andreas Bauwens

Editor: Natan T. Shaked

Chapter 7

Phase unwrapping problems in digital holographic microscopy of biological cells Alexander Khmaladze

Editor: Natan T. Shaked

Chapter 8

Partially-coherent lensless holographic microscopy on a chip and its application for automated semen analysis

Ting-Wei Su and Aydogan Ozcan

Editor: Natan T. Shaked

Chapter 9

Synthetic aperture lensless digital holographic microscopy for superresolved biological imaging

Vicente Micó, Zeev Zalevsky, Luis Granero and Javier García

Editor: Zeev Zalevsky

Chapter 10

Combining digital holographic microscopy with microfluidics: a new tool in biotechnology.

Lisa Miccio, Pasquale Memmolo, Fraancesco Merola, Melania Paturzo and Pietro Ferraro

Editor: Zeev Zalevsky

Chapter 11

Holographic motility contrast imaging of live tissues

David D. Nolte, Ran An, Kwan Jeong and John Turek

Editor: Natan T. Shaked

3. Advanced interferometric and polarization techniques

Chapter 12

Tomographic phase microscopy

Wonshik Choi

Editor: Natan T. Shaked

Chapter 13

Phase-sensitive optical coherence tomography

Itay Shock and Natan T. Shaked

Editor: Natan T. Shaked

Chapter 14

Polarization and spectral interferometric techniques for quantitative phase microscopy

Yizheng Zhu, Matthew T. Rinehart, Francisco E. Robles and Adam Wax

Editor: Natan T. Shaked

Chapter 15

Polarization microscopy Rudolf Oldenbourg Editor: Lisa L. Satterwhite

4. Phase nanoscopy

Is there a fundamental limit to optical resolution in phase microscopy? Steve Lipson

Editor: Zeev Zalevsky

Nano-holographic interferometry for in-vivo observations Federico Sciammarella, Cesar A. Sciammarella and Luciano Lamberti

Editor: Natan T. Shaked

Fluorescence phase microscopy Alberto Bilenca, Brett Bouma, Guillermo Tearney, Iwan Märki, Noelia Bocchio,

Stefan Geissbuehler and Theo Lasser

Editor: Natan T. Shaked