Dr.P.Sudha Rani is M.Sc. Life Sciences with Ph.D. specializing in Endocrinology from Sardar Patel University, Gujarat. She has a teaching experience of more than 20 years. Her area of interest is Immunology, Animal Biotechnology and Medical Biotechnology. She has guided many M.Sc., M.Phil. and Ph.D. students.

The study is focused to develop and validate HPLC methods for estimation of Allopurinol in tablet dosage form. For routine analytical purpose it is desirable to establish methods capable of analysing huge number of samples in a short time with good robustness, accuracy and precision without any prior separation steps. HPLC method generates large amount of quality data, which serve as highly powerful and convenient analytical tool. The method shows good reproducibility and good recovery. From the specificity studies, it was found that the developed methods were specific for Allopurinol. The proposed methods were found to be simple, sensitive, accurate and precise and showed no interference from the common additives and excipients. The developed method was validated in terms of linearity, accuracy, precision in accordance with the ICH guidelines. Hence the proposed methods can be routinely used for the estimation of Allopurinol in bulk and pharmaceutical dosage forms.