Chapter 1. Applications of Confocal Laser Scanning Microscopy in Filamentous Fungi

Rosa R. Mouriño-Pérez, MD, PhD and Robert W. Roberson, MD

Chapter 2. Fluorescence-based Methods for the Study of Protein Localization, Interaction, and Dynamics in Filamentous Fungi

Oier Etxebeste, PhD and Norio Takeshita, PhD

Chapter 3. Super Resolution Microscopy: SIM, STED and Localization Microscopy

James Dodgson, PhD, Anatole Chessel, PhD, Susan Cox, PhD, and Rafael Carazo Salas, PhD

Chapter 4. Fourier Transform Infrared (FTIR) Microscopy and Imaging of Fungi

Annette Naumann, Dipl. Biol.

Chapter 5. Whole Cells Imaged by Hard X-ray Transmission Microscopy

Zhiting Liang, BA, Yong Guan, MD, Shan Chen, BA, and Yangchao Tian, MD

Chapter 6. In situ Nanocharacterization of Yeast Cells Using ESEM and FIB

Yajing Shen, PhD

Chapter 7. Imaging Living Yeasts Cells and Quantifying Their Biophysical Properties by Atomic Force Microscopy

Cécile Formosa and Etienne Dague

Chapter 8. Future Directions in Mycological Microscopy

Tanya E. S. Dahms and Kirk J. Czymmek

The aim of this volume is to describe the latest advances in microscopic methods, including integrated techniques, as applied to mycology. Each chapter will provide a brief overview of a particular microscopic method with associated advantages and limitations, the research questions that can be appropriately addressed using these microscopic methods, how it has been successfully applied to address mycological research questions, including supporting and complimentary techniques, and which future questions can be addressed.

Prof. Dr. Tanya Dahms serves as professor of Biochemistry and Microscopy at the University of Regina. She is head of the Dahms group, which currently studies microbial responses to external stimuli, including mechanisms associated with carbohydrate remodeling.