Introduction: Molecular Chaperones and Protein Quality Control;Structural Disorder in Chaperone Functions Probed by NMR;Solution NMR Approaches for Studying Molecular Chaperones;Solution NMR Studies of Chaperone-Client Systems;Preparing Chaperone-Client Protein Complexes for Biophysical and Structural Studies;NMR Study of Structure and Dynamics of Chaperone-Client Complexes;Single Molecule Fluorescence Methods for Molecular Chaperones and their Client Interactions;Visualization of Chaperone Mediated Protein Folding Using X-ray Crystallography;Studying Molecular Chaperones and their Client Interactions by Nanometer Distance Restraints from Electron Paramagnetic Resonance Spectroscopy;EPR Studies of Chaperone Interactions and Dynamics;Probing Single Chaperone Substrates;Integrative Methods to Investigate Chaperones in Regulating Protein Phase Separation and Aggregation;Structural Biology in Cells by In-cell NMR