1. Historical Overview of Nanotechnology and Nanotoxicology 

2. Characterization of Nanomaterials for Toxicological Studies

3. Methods for Understanding the Interaction Between Nanoparticles and Cells

4. Single-Cell Gel Electrophoresis (COMET) Assay in Nano-Genotoxicology

5. Single-Cell Nanotoxicity Assays of Superparamagnetic Iron Oxide Nanoparticles (SPIONs)

6. Western Blot Analysis

7. Application of Reverse Transcription-PCR and Real-Time PCR in Nanotoxicity Research

8. Deriving TC₅₀ Values of Nanoparticles from Electrochemical Monitoring of Lactate Dehydrogenase (LDH) Activity Indirectly

9. Enzyme-Linked Immunosorbant Assay of IL-8 Production in Response to Silver Nanoparticles

10. Metabolomics Techniques in Nanotoxicology Studies

11. Nanoparticle Uptake Measured by Flow Cytometry

12. Determining Biological Activity of Nanoparticles as Measured by Flow Cytometry

13. Whole Cell Impedance Biosensoring Devices

14. Free Energy Calculation of Permeant-Membrane Interactions Using Molecular Dynamics Simulations

15. Screening of Fullerene Toxicity by Haemolysis Assay

16. Assessment of In Vitro Skin Irritation Potential of Nanoparticles: RHE Model

17. In Vivo Methods of Nanotoxicology

18. The Luminescent Bacteria Test to Determine the Acute Toxicity of Nanoparticle Suspensions

19. The Primacy of Physicochemical Characterization of Nanomaterials for Reliable Toxicity Assessment: A Review of the Zebrafish Nanotoxicology Model

20. Application of Embryonic and Adult Zebrafish for Nanotoxicity Assessment

21. Applications of Subsurface Microscopy 

22. Application of ICP-MS for the Study of Disposition and Toxicity of Metal-Based Nanomaterials 

23. Quantitative Nanoparticle Organ Disposition by Gel Permeation Chromatography 

24. Physiologically-Based Pharmacokinetic Modeling For Nanoparticle Toxicity Study

25. Biophysical Methods for Assessing Plant Responses to Nanoparticle Exposure

26. In Vivo Nanotoxicity Assays in Plant Models