Purification of Archaeal Chaperonin from Sulfolobus shibatae, Elsie Quaite-Randall and Andrzej Joachimiak. Purification of Hsp60 from Thermus thermophilus, Elsie Quaite-Randall and Andrzej Joachimiak. Purification of GroEL from an Overproducing E. coli Strain, Elsie Quaite-Randall and Andrzej Joachimiak. Purification of GroES from an Overproducing E. coli Strain, Elsie Quaite-Randall and Andrzej Joachimiak. Purification of the Gp31 Co-chaperonin from Bacteriophage T4, Saskia M. van der Vies. Removing Trace Fluorescent Contaminants from GroEL Preparations, Frank Weber. Assembly and Disassembly of GroEL and GroES Complexes, Jörg Martin. GroEL/GroES Interaction Assayed by Protease Protection, Jörg Martin. Determination of Chaperonin Activity In Vivo, Saskia M. van der Vies and Peter A. Lund. Interaction of Nonnative Polypeptide Substrates with the Escherichia coli Chaperonin GroEL, Wanda K. Hartmann and Edward Eisenstein. Prevention of Rhodanese Aggregation by the Chaperonin GroEL, Frank Weber and Manajit Hayer-Hartl. Refolding of Bovine Mitochondrial Rhodanese by Chaperonins GroEL and GroES, Frank Weber and Manajit Hayer-Hartl. Assay of Malate Dehydrogenase: A Substrate for the E. coli Chaperonins GroEL and GroES, Manajit Hayer-Hartl. Assay of Chaperonin-Assisted Refolding of Citrate Synthase, N. Kalaya Steede, Stacy L. Temkin, and Samuel J. Landry. Purification of Yeast Mitochondrial Hsp60, Yves Dubaquié, Renate Looser, and Sabine Rospert. Preparation of Recombinant Hsp10, N. Kalaya Steede, Jesse J. Guidry, and Samuel J. Landry. Purification of the Cytosolic Chaperonin TRiC from Bovine Testis, Raul G. Ferreyra and Judith Frydman. Monitoring Actin Folding: Purification Protocols for Labeled Proteins and Binding to DNase I-Sepharose Beads, Vanitha Thulasiraman, Raul G. Ferreyra, and Judith Frydman. Folding Assays: Assessing the Native Conformation of Proteins, Vanitha Thulasiraman, Raul G. Ferreyra, and Judith Frydman. Purification of Prefoldin, Sally A. Lewis andNicholas J. Cowan. Purification of GimC from Saccharomyces cerevisiae, Katja Siegers and Elmar Schiebel. Analysis of Eukaryotic Molecular Chaperone Complexes Involved in Actin Folding, Michel R. Leroux. Index

In Chaperonin Protocols, Christine Schneider has assembled a unique collection of readily reproducible protocols for the study of chaperonins, intracellular proteins critical to many biological processes. Written by experienced investigators who have successfully honed their methods to a fineness, the protocols focus on the purification of chaperonins from different species along with their corresponding cofactors, and on chaperonin activity assays for in vivo as well as in vitro work. Many activity assays are given for GroEL, which can also be applied to mitochrondrial Hsp60. There are also assays for the eukaryotic chaperonin TRiC and handy methods-for example, one for preparing labeled probes-that can be used for various purposes and prove helpful in numerous different procedures.

Critically important to a greater understanding of such disorders as cystic fibrosis, Alzheimer's disease, and BSE, Chaperonin Protocols offers both novice and experienced investigators fast access to today's best and most productive chaperonin methods, all explained in step-by-step detail to ensure robust and reproducible results.