Over the past ten years, many powerful new techniques have been developed that have dramatically changed malaria research. The second edition of  Malaria: Methods and Protocols expands upon the previous edition with current, detailed techniques for laboratory research. With new chapters on parasite culture techniques, genome manipulation methods, 'omic' approaches, and  techniques for studying the biology of the red blood cell and pre-erythrocytic stages of Plasmodium. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
 
Authoritative and practical,  Malaria: Methods and Protocols, Second Edition, offers a comprehensive set of standard techniques for laboratory  researchers.

In vitro Culturing
P. falciparum
Erythrocytic Stages.-Production of
Plasmodium falciparum
Gametocytes
in vitro
.-
In vitro
Differentiation of
Plasmodium falciparum
Gametocytes into Ookinetes.-
Ex vivo
culture of
Plasmodium Vivax
and
Plasmodium Cynomolgi
and
in vitro
Culture of
Plasmodium Knowlesi
Blood Stages.-Laboratory Maintenance of Rodent Malaria Parasites.-Transfection of
Plasmodium falciparum
.-Transfection of Rodent Malaria Parasites.-Recombination-mediated Genetic Engineering of
Plasmodium berghei
DNA.-Standardization in Generating and Reporting Genetically Modified Rodent Malaria Parasites: The RMgmDB Database.-Transient Transfection of
Plasmodium vivax
Blood-stage Parasites.-Silencing of Genes and Alleles by RNAi in
Anopheles gambiae
.-Analysis of Variant Gene Family Expression by Quantitative PCR.-DNA Microarray-based Genome-wide Analyses of
Plasmodium
Parasites.-Whole-genome Analysis of
Plasmodium
spp
.
Utilizing a New Agilent Technologies DNA Microarray Platform.-Transcriptome Analysis using RNA-seq.-Experimental Tools for the Study of Protein Phosphorylation in
Plasmodium
.-Extraction of Hydrophilic Metabolites from
Plasmodium falciparum
-infected Erythrocytes for Metabolomic Analysis.-Static and Dynamic Imaging of Erythrocyte Invasion and Early Intra-erythrocytic Development in
Plasmodium falciparum
.-Flow Cytometry-based Methods for Measurement of Cytosolic Calcium and Surface Protein Expression in
Plasmodium falciparum
Merozoites.-Microsphiltration: a Microsphere Matrix to Explore Erythrocyte Deformability.-Atomic Force Microscopy of
Plasmodium
-infected Red Blood Cells for Detecting and Localizing Single Molecular Recognition Events.-Expressing Full-length Functional PfEMP1 Proteins in the HEK293 Expression System.-Genome-wide Chromatin Immunoprecipitation-sequencing in
Plasmodium
.-
In situ
Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of
Plasmodium falciparum
.-Bioluminescence Imaging of
P. berghei
Schizont Sequestration in Rodents.-Scoring Sporozoite Motility.-Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry.-Imaging Sporozoite Cell Traversal in the Liver of Mice.-Analysis of Liver Stage Development in, and Merozoite Release from Hepatocytes.-Quantitative Analysis of
Plasmodium berghei
Liver Stages by Bioluminescence Imaging.-Activation of Human NK Cells by
Plasmodium
-infected Red Blood Cells.-Chimeric Parasites as Tools to Study
Plasmodium 
Immunology and Assess Malaria Vaccines.-Development and Use of TCR Transgenic Mice for Malaria Immunology Research.-Tracking the Total CD8 T Cell Response Following Whole
Plasmodium
Vaccination .-Screening Inhibitors of
P. berghei
Blood Stages using Bioluminescent Reporter Parasites.-Screening and Evaluation of Inhibitors of
Plasmodium falciparum
Merozoite Egress and Invasion using Cytometry.-Induction of anti-
Plasmodium
Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug Cure.-Vaccination using Radiation-or Genetically-Attenuated Live Sporozoites.-Vaccination using Normal Live Sporozoites Under Drug Treatment.-Assessing Transmission Blockade in
Plasmodium
spp. .-Mosquito Transgenic Technologies to Reduce
Plasmodium
Transmission..-Silencing of Genes and Alleles by RNAi in
Anopheles gambiae
.-Analysis of Variant Gene Family Expression by Quantitative PCR.-DNA Microarray-based Genome-wide Analyses of
Plasmodium
Parasites.-Whole-genome Analysis of
Plasmodium
spp
.
Utilizing a New Agilent Technologies DNA Microarray Platform.-Transcriptome Analysis using RNA-seq.-Experimental Tools for the Study of Protein Phosphorylation in
Plasmodium
.-Extraction of Hydrophilic Metabolites from
Plasmodiumfalciparum
-infected Erythrocytes for Metabolomic Analysis.-Static and Dynamic Imaging of Erythrocyte Invasion and Early Intra-erythrocytic Development in
Plasmodium falciparum
.-Flow Cytometry-based Methods for Measurement of Cytosolic Calcium and Surface Protein Expression in
Plasmodium falciparum
Merozoites.-Microsphiltration: a Microsphere Matrix to Explore Erythrocyte Deformability.-Atomic Force Microscopy of
Plasmodium
-infected Red Blood Cells for Detecting and Localizing Single Molecular Recognition Events.-Expressing Full-length Functional PfEMP1 Proteins in the HEK293 Expression System.-Genome-wide Chromatin Immunoprecipitation-sequencing in
Plasmodium
.-
In situ
Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of
Plasmodium falciparum
.-Bioluminescence Imaging of
P. berghei
Schizont Sequestration in Rodents.-Scoring Sporozoite Motility.-Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry.-Imaging Sporozoite Cell Traversal in the Liver of Mice.-Analysis of Liver Stage Development in, and Merozoite Release from Hepatocytes.-Quantitative Analysis of
Plasmodium berghei
Liver Stages by Bioluminescence Imaging.-Activation of Human NK Cells by
Plasmodium
-infected Red Blood Cells.-Chimeric Parasites as Tools to Study
Plasmodium 
Immunology and Assess Malaria Vaccines.-Development and Use of TCR Transgenic Mice for Malaria Immunology Research.-Tracking the Total CD8 T Cell Response Following Whole
Plasmodium
Vaccination .-Screening Inhibitors of
P. berghei
Blood Stages using Bioluminescent Reporter Parasites.-Screening and Evaluation of Inhibitors of
Plasmodium falciparum
Merozoite Egress and Invasion using Cytometry.-Induction of anti-
Plasmodium
Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug Cure.-Vaccination using Radiation-or Genetically-Attenuated LiveSporozoites.-Vaccination using Normal Live Sporozoites Under Drug Treatment.-Assessing Transmission Blockade in
Plasmodium
spp. .-Mosquito Transgenic Technologies to Reduce
Plasmodium
Transmission.