Three Phase Partitioning: Applications in Separation and Purification of Biological Molecules and Natural Products presents applications in diverse areas of both chemical technology and biotechnology. This book serves as a single resource for learning about both the economical, facile and scalable processes, along with their potential for applications in the separation and purification of materials and compounds across the entire spectra of chemical and biological nature. The book begins by explaining the origins and fundamentals of TPP and continues with chapters on related applications, ranging from the purification of parasite recombinant proteases to oil extraction from oilseeds and oleaginous microbes, and more.

• Written by researchers who have been pioneers in developing and utilizing three phase partitioning
• Focuses on applications, with chapters detailing relevance to a wide variety of areas and numerous practical examples
• Designed to give laboratory workers the information needed to undertake the challenge of designing successful three-phase partitioning protocols

1. The origins of Three Phase Partitioning 2. Three Phase Partitioning: How it got applied to areas other than protein concentration and purification 3. The multiple facets of three phase partitioning in the purification, concentration and preservation of proteins 4. Enzymes recovery by Three Phase Partitioning: an overview 5. Emulsion gel formation in three phase partitioning 6. Using three phase partitioning to purify parasite recombinant proteases 7. Partitioning of plant peroxidases by Three Phase Partitioning 8. Process optimization and purification of naringinase and asparaginase enzymes using three phase partitioning (TPP) 9. Macro-[affinity ligand] facilitated three phase partitioning [MLFTPP]. Converting Three Phase Partitioning into an affinity-based process 10. Applications of Three Phase Partitioning and Macro-[affinity ligand] facilitated three phase partitioning (MLFTPP) in protein refolding 11. Three phase partitioning-based strategies for highly efficient separation of bioactive polysaccharides from natural resources 12. Using Three Phase Partitioning to isolate carbohydrates of different molecular weights 13. Technologies for oil extraction from oilseeds and oleaginous microbes: A comprehensive review 14. Three Phase Partitioning as an extraction technique for oleageneous materials 15. Three phase partitioning as a method for isolation of bioactive compounds from plant and animal tissues 16. Three-phase partitioning for simultaneous extraction and purification of biomolecules